Chromatography

  1 Introduction of Column with Polymer Matrix Polymer matrix columns (Sugar-H, Sugar-Ca) may be contaminated, resulting in decreased column efficiency. Thus, flushing and regeneration are required. However, when flushing these columns with strong organic solvent, the column packing materials which exposed to some organic solvents will swell and column bed will be damaged. The swelling degree of the packing materials depends on the cross-linking degree of the polymer. Generally, the polymer whose cross-linking degree is higher than 8-10% has better physical stability. The shrinkage in the aqueous solution and the expansion in the organic solvent are both small, but limitation of using condition still need to be paid attention. 02 Daily Flushing  03 Regeneration and Flushing 04 Precaution 1. There is no need to worry that 85 °C is so high that it will damage the column. Activation and use of polymer matrix column at 85 °C is better than at 50-60 °C. 2. Before the column temperature reac

  Blossmate TM  PSV C18        Blossmate TM  PSV C18 is a brand new column ● Super high purity spherical silica matrix ● Bonded high-density alkyl functional groups ● Strong retention ability and selectivity ●Can be more perfectly compatible with the high proportion of aqueous phase ●Keep stable baselines and high sensitivity even at neutral pH condition It shows full compatibility when used with technologies such as LC-MS and can be widely used in the analysis of various components such as oligosaccharides, amino acids, small peptides, nucleotides and organic acids. It is a dedicated column designed for the detection of food additives Other common C18 column analysis of food additives, either dehydroacetic acid and saccharin sodium are too close, or dehydroacetic acid tailing is undesired. Blossmate TM  PSV C18 has stronger retention ability and is comparable to other C18 reversed-phase columns. The improved tailing and excellent resolution are highly appreciated. Column： Blossmate TM

  When the injection volume of test substance within the range of the security, changing injection volume will affect the peak height and peak area accordingly. But the influence of retention time, peak width, and resolution is slight. When injection volume or the quality is excessive, it will cause the overload phenomenon. With the peak width increases, poor peak shape and lower resolution is inevitable. Overload When the injection volume of test substance within the range of the security, changing injection volume will affect the peak height and peak area accordingly. But the influence of retention time, peak width, and resolution is slight. When injection volume or the quality is excessive, it will cause the overload phenomenon. With the peak width increases, poor peak shape and lower resolution is inevitable. For columns whose length is 50 to 250mm and inner diameter is 4 to 5mm, injection quality of a single sample injection should be less than 50 μg and the injection volume shoul