Flushing and Regeneration of Column with Polymer Matrix

 

1

Introduction of Column with Polymer Matrix

Polymer matrix columns (Sugar-H, Sugar-Ca) may be contaminated, resulting in decreased column efficiency. Thus, flushing and regeneration are required. However, when flushing these columns with strong organic solvent, the column packing materials which exposed to some organic solvents will swell and column bed will be damaged. The swelling degree of the packing materials depends on the cross-linking degree of the polymer. Generally, the polymer whose cross-linking degree is higher than 8-10% has better physical stability. The shrinkage in the aqueous solution and the expansion in the organic solvent are both small, but limitation of using condition still need to be paid attention.

02

Daily Flushing 

03

Regeneration and Flushing

04

Precaution

1. There is no need to worry that 85 °C is so high that it will damage the column. Activation and use of polymer matrix column at 85 °C is better than at 50-60 °C.

2. Before the column temperature reaches the set value, please set the flow rate at 0.2~0.3ml/min to avoid too high column pressure.

3. When the column temperature reaches the set value, the flow rate increases to the analytical flow rate and the change of flow rate keeps an increment of 0.1ml/min. After the column pressure is stabilized, continue to increase the flow rate so as to avoid sharp increase of the flow rate that will lead to excessive pressure pulses and damage of the column. Similarly, the flow rate should be reduced at 0.1ml/min each time.

4. The maximum flow rate shall not exceed 14 MPa.

5. After use, the pump can only be stopped after the column temperature is lowered to room temperature. Take off the column and store at 4℃ in the refrigerator. After taking it out of the refrigerator, it should resume to room temperature to be placed in column oven.

6. The maximum content of organic phase in mobile phase is 5% (V/V). A small amount of acetonitrile, ethanol, methanol and isopropanol in the sample will not affect the properties of the column.

7. Sufficient degassing of mobile phase ensures great baseline.

If you have any problem or require further information, please contact info@welchmat.com.