Firstly, let us learn something about the adsorption mechanism of reversed-phase extraction and normal phase extraction. Reversed-phase extraction separation mainly uses the non-polar force between C-H bonds on SPE functional groups and C-H bonds of target compounds. In general, non-polar reversed-phase SPE columns are more suitable for extraction and separation of non-polar target compounds or target compounds with moderate polarity from polar matrices. Target compounds adsorbed on nonpolar SPE columns by nonpolar forces can be eluted with nonpolar solvents, such as chloroform, cyclohexane, and ethyl acetate. The target compound can be eluted from the SPE column successfully as long as the solvent elution strength is sufficient to destroy the van der Waals force between the target compound and the non-polar functional group of the adsorbent. Even methanol with strong polarity has sufficient nonpolar force to elute many compounds. Sometimes s...
When you look up the pharmacopoeia method, you may find that it only stipulates the bonding phase of column and the information of other columns is not available. Therefore, you may feel confused about how to choose a suitable column. Welch Materials, as the most professional chromatographic consumables manufacturers in China, would like to share with you some experience as follows. 01 Basic Knowledge of Column Chromatography is a separation analysis method, separation is the core, so the column which can achieve separation is like the heart of the chromatographic system. Common requirements for column are high column efficiency, good selectivity, fast analysis speed, etc. Packing materials for HPLC are mainly all-porous spherical silica gel, organic polymer matrix, core-shell silica gel, etc. The particle size is generally 3,5,7,10 µm, etc. And theoretical number of plates can reach 80,000 / m. 02 Selection of S...
Do you still feel tired about complicated and time-consuming sample preparation? In pursuit of more efficient and faster sample pretreatment, Welch launched a new generation of product- SinCHERS pretreatment purification column. Compared with traditional SPE technology, SinCHERS column greatly improved detection efficiency. Compared with the QuEChERS, its purification process does not require centrifugation again and only need one step (30s) to complete the purification process. The development of electric supporting equipment enables the whole purification process to be semi-automated. The purification of 12 samples can be completed within 3 minutes, which greatly improves the detection efficiency and relatively reduces the detection cost of the laboratory. SinCHERS: Meet AOAC 2007.1 and EN 15662 standards ● ● SinCHERS=Single-step +Cheap+Effective+Rugged+Safe QueChERS=Quick+Easy+Cheap+Effective+Rugged+Safe / / Perfect su...
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