Firstly, let us learn something about the adsorption mechanism of reversed-phase extraction and normal phase extraction. Reversed-phase extraction separation mainly uses the non-polar force between C-H bonds on SPE functional groups and C-H bonds of target compounds. In general, non-polar reversed-phase SPE columns are more suitable for extraction and separation of non-polar target compounds or target compounds with moderate polarity from polar matrices. Target compounds adsorbed on nonpolar SPE columns by nonpolar forces can be eluted with nonpolar solvents, such as chloroform, cyclohexane, and ethyl acetate. The target compound can be eluted from the SPE column successfully as long as the solvent elution strength is sufficient to destroy the van der Waals force between the target compound and the non-polar functional group of the adsorbent. Even methanol with strong polarity has sufficient nonpolar force to elute many compounds. Sometimes s...
CPhI & P-MEC India, an event known by all the pharmaceuticals companies for the largest Pharmaceutical trade fair in the India, held at India Expo Mart, Greater Noida (Delhi-NCR). As this event is a gateway to successfully grow your business and unlock the potential in the pharma market, Welch Materials India also being a part of this three days event placed between 24th Nov to 26th November 2021 featured for Analytical equipment's in all ranges from Chromatography consumables (HPLC Columns, Vials, Syringe Filters, SPE, Quechers, Caps & Septa) to the DAC (Dynamic Axial Compression Column) and HPLC system. As Welch Materials products are running very successfully through assurance of quality and feasibility around all over the India and World, many clients from top notched pharmaceuticals companies from India and abroad shared their valuable time on our booth for more reach of our product line, applications and to avail our services. Welch Materials would like to tha...
When you look up the pharmacopoeia method, you may find that it only stipulates the bonding phase of column and the information of other columns is not available. Therefore, you may feel confused about how to choose a suitable column. Welch Materials, as the most professional chromatographic consumables manufacturers in China, would like to share with you some experience as follows. 01 Basic Knowledge of Column Chromatography is a separation analysis method, separation is the core, so the column which can achieve separation is like the heart of the chromatographic system. Common requirements for column are high column efficiency, good selectivity, fast analysis speed, etc. Packing materials for HPLC are mainly all-porous spherical silica gel, organic polymer matrix, core-shell silica gel, etc. The particle size is generally 3,5,7,10 µm, etc. And theoretical number of plates can reach 80,000 / m. 02 Selection of S...
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